Firsttime users of bd facsdiva software should read. Our selfpaced elearning courses can be used for basic learning or to refresh and. Bd facs canto ii flow cytometer quick user guide v. To sign up for visit 2, please fill out a training request form. History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4.
A june 2007 bd biosciences benex limited canada japan bay k 1 ad nippon becton dickinson 2350 qume drive toll free 888 2590187 company, ltd. Ddx4positive and negative cells, for both human ovarian cells and transfected hek 293 t cells, were sorted using an aria iiu cytometer equipped with a 488 nm laser, running bd facsdiva v6 software bd biosciences, oxford, uk. Learn more about reagent selection and instrument setup with our application notes and technical bulletins. Bd biosciences will release a version of bd facsdiva software to correct this problem. Using templates in bd facsdiva software selfpaced bd facsvia online training program. Running a basic 2 color flow cytometry experiment in bd facs diva. Bd facsdiva software reference manual for version 8. Page 1 bd facscanto ii instructions for use for in vitro diagnostic use part no.
Please be sure to bring the flow cores facsdiva software guide with you for this visit. Please note, this guide is not intended to take the place of inperson training but is to be used as a reference. Refer to the information supplied by the manufacturer for applicationspecific limitations. User are asked to contact bd customer support center at 18772328995 if they have any additional questions. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments.
However, diva has come along way over the past 10 years and many improvements have been made to help enduser. To help customers, take full and immediate advantage of their bd products capabilities, bd biosciences offers training for instruments, software, and applications. Replace the waste container if indicator is on see below. This video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Interference there are no known incompatibilities with bd facscanto clinical. Were here to help you accelerate routine phenotyping, take your immunology research to the next level, and get you from data to results. Standard lsr training training flow cytometry shared. Bd facsdiva software quick reference guide for the bd lsr ii. Class 2 device recall bd biosciences facsdiva software. In the login window, scroll down through the list of user names to locate yours. Small classes offer personalized instruction on the proper use and maintenance of the bd lsr ii flow cytometer, as well as how to best use bd facsdiva software on the bd lsr ii flow cytometer for maximum performance. It is not intended to teach flow cytometry theory or best practices. Bd facsdiva software contains vxworks as embedded software runtime.
The software adds new analysis capabilities and enhances integration with bd facsdiva software, making it easier for users to analyze multiple flow cytometry runs and. Bd facsdiva software contains vxworks as embedded software runtime module. Catalog number 641609 flexible data acquistion and analysis package specifically designed for digitalbased flow cytometers. This program is intended for laboratories and individuals who have a need to sort regularly after normal core facility hours, 8 am 5 pm mondayfriday. Becton dickinson bd facsdiva software is a technology used for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows in a laboratory and healthcare setting. Bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. Bd facsdiva v6 software is a collection of rich tools for flow cytometer and application setup, data acquisition, and analysis. Written by tim bushnell, phd bd biosciences brand of digital flow cytometers, including. Bd biosciences is not liable for any claims related to or resulting from buyerusers failure to install and maintain virus protection. The name of the program executable file is bd facsdiva software. In spectral flow, light is collected in all detectors for all parameters and the additional information allows software to separate out the individual colors more precisely. Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument.
Not for use in diagnostic or therapeutic procedures. This visit is for one trainee at a time with no exceptions. Page 1 bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. Compensation of a 7 color panel on the bd lsr ii youtube. Bd biosceinces instrument and application trainings. Candidates should be familiar with the facsdiva program and have done their preliminary analysis work on an lsr. Computer and software login to log into the computer, use lsrii as the user name and lsrii as the password. Bd biosciences brand of digital flow cytometers, including the facscanto, the lsrii, facsaria and fortessa, utilize a software acquisition program known as facsdiva. Refill as necessary from large plastic canister containing 1x sheath solution. Courses are led by professional instructors with extensive flow cytometry experience gained in research and clinical laboratories.
Running beads allows the user to begin to learn the software. To log into the computer, use lsrii as the user name and lsrii as the password. The bd facsdiva option was designed as an integrated hardware software platform and introduces a number of additional benefits not directly related to the digital nature of the system. Using application settings to standardize flow cytometry. Please contact us to schedule time on the instrument, set up a training appointment or to discuss a future project. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. Accelerate your discovery with the latest features that come with flowjo v10. Flowjo is your biggest fan and strives to be an outstanding source of support. The runtime module was developed by a third party and we are. The new digital acquisition platform introduces a complete new software concept from bd. In january 2018, bd was made aware of some antivirus vendors flagging an installed file in the bd facsdiva software. Diva is aptly named as it can be a difficult program to master. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Biexponential scaling in the bd facsdiva software reference manual.
University of massachusetts medical school flow cytometry. The wide range of product courses includes handson, instructorled training, as well as elearning in instructor led virtual classrooms, selfpaced study, and online webinars. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and. This is a 4laser instrument equipped with digital bd facsdiva software. Product security service bulletin false positive file. Bd customer education provides comprehensive training on bd instruments and software. Capabilities provided by this instrument include quantification of cellular subsets based on the expression of surface or intracellular antigens, analysis of apoptosis and cell cycle parameters, and multiparameter analyses.
The software runs on a windows2000 computer, macintosh systems are no longer available. That will be discussed during your instrument training, but is not included here. Bd biosciences facsdiva software features bd facsdiva. Follow only those sections that apply to the software you are running. The software uses a database server software to manage the flow.
Verify the settings after defining the baseline and when you change reagent lots. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Bd facsdiva software basic experiment guide please note, this guide is not intended to take the place of inperson training but is to be used as a reference as needed after completing training with unified flow core staff. Preparing a facsdiva experiment this document is intended to be a software guide only. Most software functions are controlled using the tool bar at the top of the workspace and toolbars within the browser and worksheet windows. The most popular version of this product among our users is 4. Mcbc flow cytometry training course session 1 duration.
Regarding this runtime module, you are prohibited from. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. Bd facscanto clinical software set up the cytometer with bd facscanto clinical software, even if you. To practice using the instrument setup feature, do the corresponding tutorial in. Bd facscanto ii quick reference manual pdf download. For the demonstration, we will assume this is a 4color flow cytometric analysis experiment. This operator course is a practical oriented training course that combines general and. Spectral compensation allows users who collect more channels than they have parameters to get cleaner data if properly done.
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